FastQCFastQC Report
Thu 2 Jun 2016
DRR015417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172625
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151430.16508905575012606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122370.13340783036480833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148950.050.3964961
GTATCAA283250.040.5553741
TCAACGC328150.034.4515344
ATCAACG328650.034.3852773
CAACGCA343750.032.9277655
TATCAAC352750.032.177932
AACGCAG357400.031.707326
ACGCAGA396100.027.931817
CGCAGAG394650.027.5603148
GTACATG323850.024.4574851
GCAGAGT423450.024.332019
TACATGG320200.023.3153442
GTGGTAT69600.022.6164171
ACATGGG339450.021.4570543
TGGTATC75050.020.2465172
GAGTACT209250.019.82579812-13
CAGAGTA409500.019.6953310-11
AGAGTAC374800.019.32806210-11
CATGGGG200650.018.7508494
GTACTTT216000.018.7006614-15
Produced by FastQC (version 0.11.5)