FastQCFastQC Report
Thu 2 Jun 2016
DRR015417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172625
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113320.12354151619629059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121500.053.6119581
GTATCAA226750.047.0537151
ATCAACG284150.036.8949973
TATCAAC295850.035.50952
TCAACGC301950.034.129324
CAACGCA323150.031.9837175
AACGCAG337150.030.749566
ACGCAGA379150.026.5442337
CGCAGAG381300.025.8773048
GTACATG280650.025.0146621
TACATGG284200.023.5963842
GCAGAGT408500.022.845719
ACATGGG301250.021.4477863
TATAACG6650.021.2819042
GTGGTAT63700.020.6910571
AGAGTAC370500.019.16049410-11
GAGTACT216100.018.79706812-13
TGGTATC67850.018.2912672
GTACTTT219150.018.08956114-15
CAGAGTA410150.017.84892310-11
Produced by FastQC (version 0.11.5)