FastQCFastQC Report
Thu 2 Jun 2016
DRR015416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19683097
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383940.195060767114037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254520.12930891922140098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266450.050.2328151
GTATCAA528750.044.3976361
TCAACGC628100.036.860194
ATCAACG628650.036.755483
CAACGCA660950.035.014345
TATCAAC666550.034.8703232
AACGCAG688850.033.7538266
ACGCAGA766500.029.5509727
CGCAGAG766750.028.9819978
GCAGAGT831200.025.4209759
GTACATG599550.025.3747311
TACATGG599800.023.9908472
ACATGGG627950.021.9010983
GTGGTAT120450.021.755861
AGAGTAC745000.019.80791510-11
GAGTACT473750.019.63116312-13
CAGAGTA802250.019.53127910-11
TGGTATC130250.018.5468252
CATGGGG379650.018.4660134
GTACTTT494700.018.3857614-15
Produced by FastQC (version 0.11.5)