FastQCFastQC Report
Thu 2 Jun 2016
DRR015416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19683097
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330480.16790040713613308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201590.10241782581267571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219800.054.367841
GTATCAA453750.053.943641
ATCAACG574350.042.1094783
TATCAAC596450.040.676872
TCAACGC615250.038.72714
CAACGCA666750.035.754885
AACGCAG697800.034.234976
ACGCAGA778300.029.985447
CGCAGAG782300.029.3392528
GCAGAGT841150.025.9991449
GTACATG526050.025.5613161
TACATGG541350.023.54882
ACATGGG571500.021.5070843
AGAGTAC770750.020.54646310-11
GAGTACT502150.019.8648812-13
CAGAGTA824050.019.05823110-11
GTGGTAT111650.019.0570431
AGTACTT523400.018.9416312-13
GTACTTT525350.018.57682614-15
CATGGGG356400.017.4861284
Produced by FastQC (version 0.11.5)