FastQCFastQC Report
Thu 2 Jun 2016
DRR015415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13515967
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414020.30631918530135505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318000.2352772835269574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167580.12398668922467773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225200.056.198521
GTATCAA455950.043.5215761
TCAACGC544550.036.0137524
ATCAACG545750.035.9843143
CAACGCA569550.034.352835
TATCAAC590950.033.4013372
AACGCAG594050.033.0422676
ACGCAGA650750.029.5411477
CGCAGAG657300.028.9909218
GTGGTAT84450.026.3417951
GCAGAGT699850.026.1947179
GTACATG448350.024.2299941
TACATGG438300.023.0620292
TGGTATC93750.022.3694112
ACATGGG455550.021.5596393
CAGAGTA683550.021.15601510-11
GTACTTT443200.020.50643314-15
GAGTACT451500.020.42204312-13
AGAGTAC647750.020.1622710-11
AGTACTT449350.018.80893312-13
Produced by FastQC (version 0.11.5)