FastQCFastQC Report
Thu 2 Jun 2016
DRR015415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13515967
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346920.2566741987458241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242820.17965418234596162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162110.11993962400174549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181900.063.594621
GTATCAA376800.052.1644481
ATCAACG497650.039.0888253
TATCAAC522900.037.3261952
TCAACGC530500.036.144614
CAACGCA569500.033.708545
AACGCAG597850.032.20086
ACGCAGA656050.028.6958737
CGCAGAG663700.028.0618138
GTGGTAT75600.026.0066241
GCAGAGT702550.025.5684229
GTACATG394100.024.4190461
TGGTATC83750.022.875992
TACATGG399950.022.1957762
AGAGTAC673800.020.74515310-11
ACATGGG416100.020.5102233
CAGAGTA707150.019.8691910-11
GTACTTT462100.019.70395914-15
AGTACTT468900.019.67088912-13
GAGTACT474950.019.51560812-13
Produced by FastQC (version 0.11.5)