FastQCFastQC Report
Thu 2 Jun 2016
DRR015414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9236094
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280850.3040787588346329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224060.2425917276285841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107560.11645615559997549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205450.044.4196241
GTATCAA353950.037.389691
TCAACGC409450.031.8428044
ATCAACG410250.031.7472693
CAACGCA422300.030.8523255
TATCAAC428100.030.6148172
AACGCAG437300.029.86596
ACGCAGA487150.026.4175247
CGCAGAG486050.026.1398128
TATAACG8400.024.9122052
GCAGAGT511150.023.8593469
GTACATG271100.023.0740181
TACATGG275850.021.7688052
GAGTACT293350.021.71718812-13
AGAGTAC425450.020.17598210-11
ACATGGG286550.020.0351493
CAGAGTA496300.019.69256810-11
ATAACGC10650.019.6490633
GTACTTT323800.019.56231514-15
TAACGCA10950.018.6953834
Produced by FastQC (version 0.11.5)