FastQCFastQC Report
Thu 2 Jun 2016
DRR015414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9236094
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217080.23503442039459538No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163810.1773585240687243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96740.10474124667852015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180400.043.917861
GTATCAA305300.042.6449361
TATCAAC377950.034.1231082
ATCAACG376900.034.0593533
TCAACGC398400.031.9692154
CAACGCA421550.030.185475
AACGCAG436850.029.2109836
ACGCAGA484200.025.9344487
CGCAGAG485800.025.554698
GCAGAGT507750.023.468079
GTACATG235650.022.829911
GAGTACT294450.021.11828612-13
TACATGG243500.021.0963632
AGAGTAC426250.020.87904710-11
ACATGGG252600.019.4961933
AGTACTT314150.019.45543912-13
GTACTTT323450.018.85963414-15
CAGAGTA510650.018.27897310-11
TATAACG7700.016.9611432
TACTTTT386700.015.7339314-15
Produced by FastQC (version 0.11.5)