FastQCFastQC Report
Thu 2 Jun 2016
DRR015413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12520423
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196820.15719916172161277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.11734427822446573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177700.054.2478641
GTATCAA350300.044.3106351
TCAACGC423450.036.086684
ATCAACG424200.036.0118683
TATCAAC455550.033.5535772
CAACGCA456700.033.429385
AACGCAG473650.032.3183566
ACGCAGA505750.029.2597037
CGCAGAG508100.028.505938
GCAGAGT552300.025.1123189
GTACATG453600.023.8293651
TACATGG449650.022.6322142
ACATGGG469400.021.0306283
CAGAGTA530900.020.0882810-11
AGAGTAC500100.019.7791610-11
GTGGTAT75200.019.358481
GAGTACT273800.018.88144312-13
GTACTTT267150.018.62745514-15
CATGGGG297950.018.154224
AGTACTT270500.017.93464512-13
Produced by FastQC (version 0.11.5)