FastQCFastQC Report
Thu 2 Jun 2016
DRR015413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12520423
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155440.12414916013620307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142300.057.8600771
GTATCAA276000.051.71711
ATCAACG370250.038.1780933
TATCAAC386550.036.70892
TCAACGC393300.035.39184
CAACGCA429150.032.511535
AACGCAG448450.031.1625376
ACGCAGA492350.027.6583617
CGCAGAG494900.027.043798
GTACATG399250.026.033551
TACATGG408500.024.3381252
GCAGAGT535050.023.7019029
ACATGGG430150.022.5062473
AGAGTAC491200.020.23407710-11
GTGGTAT69000.018.5337681
AGTACTT281100.018.40326112-13
CAGAGTA528350.018.31451410-11
CATGGGG289100.018.2181724
GAGTACT279500.017.96614612-13
GTACTTT274700.017.68514314-15
Produced by FastQC (version 0.11.5)