FastQCFastQC Report
Thu 2 Jun 2016
DRR015412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428786
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129560.29254066464263573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96850.21868295284531697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.12793573679107548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95400.039.1495481
GTATCAA161000.035.3196261
TCAACGC186100.030.1400364
ATCAACG187650.030.1335033
CAACGCA193100.029.0709975
TATCAAC197000.028.7725832
AACGCAG200800.028.0455766
GTACATG124450.026.6479991
TACATGG123450.025.5738092
ACGCAGA221850.024.7488637
CGCAGAG222350.024.42678
ACATGGG128650.023.7621943
GCAGAGT239300.021.8981519
GAGTACT140750.020.11817612-13
AGAGTAC191200.019.99631310-11
CATGGGG77150.019.1634854
AGTACTT147050.018.3127812-13
GTACTTT152900.018.10270114-15
CAGAGTA231600.017.84376310-11
CATGGGA51000.017.1260854
Produced by FastQC (version 0.11.5)