FastQCFastQC Report
Thu 2 Jun 2016
DRR015412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4428786
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116190.2623518047609435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88300.199377436615813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57320.12942598716668632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95100.044.0448651
GTATCAA158750.041.265791
TATCAAC197350.033.0062182
ATCAACG195700.033.0052723
TCAACGC206800.030.5467454
CAACGCA220900.028.6157675
AACGCAG227700.027.8406756
TAGCGCG858.5081765E-426.6195019
ACGCAGA247100.025.3075317
CGCAGAG247200.025.1170838
GCAGAGT260150.022.8396669
GTACATG113400.022.7119751
GAGTACT154700.021.69050612-13
AGAGTAC213700.021.46189510-11
TACATGG112850.021.3062862
ACATGGG114900.020.3078773
AGTACTT162350.020.26426912-13
GTACTTT163050.020.25824514-15
TTTCGCG2853.596142E-919.0539579
CAGAGTA258300.018.3342710-11
Produced by FastQC (version 0.11.5)