FastQCFastQC Report
Thu 2 Jun 2016
DRR015411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9003566
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297790.33074672857398946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242920.26980420868797983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118220.1313035301790424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169100.056.4674841
GTATCAA323650.042.102971
ATCAACG382000.035.2303773
TCAACGC383050.035.205094
CAACGCA396350.034.000785
TATCAAC406650.033.3307652
AACGCAG412900.032.671156
ACGCAGA458600.028.840027
CGCAGAG459250.028.3732078
GCAGAGT491000.025.8044439
GTGGTAT65450.025.7988761
GTACATG286800.022.8676411
TGGTATC70500.022.5954152
GAGTACT311200.022.2058712-13
TACATGG282650.021.9154572
CAGAGTA476100.021.55239310-11
GTACTTT329150.020.56636214-15
AGAGTAC448950.020.3730910-11
ACATGGG295200.020.135213
AGTACTT319850.019.09490612-13
Produced by FastQC (version 0.11.5)