FastQCFastQC Report
Thu 2 Jun 2016
DRR015411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9003566
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306500.34042067331988235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242810.26968203487373776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136190.15126228874203843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161700.057.880681
GTATCAA324300.043.53581
ATCAACG396600.035.0721243
TCAACGC397350.035.028254
CAACGCA407000.034.2545475
TATCAAC419600.033.335012
AACGCAG424900.032.810036
ACGCAGA480750.028.6299367
CGCAGAG480000.028.3308628
GTGGTAT63350.025.737611
GCAGAGT516100.025.6609279
TGGTATC66050.023.6371352
GAGTACT337400.021.5827812-13
GTACATG261650.021.2359121
CAGAGTA499850.020.99675210-11
AGAGTAC472400.020.9354510-11
GTACTTT354100.020.18528414-15
TACATGG265050.020.0923882
AGTACTT347150.019.98677412-13
TATAACG8450.019.3918532
Produced by FastQC (version 0.11.5)