FastQCFastQC Report
Thu 2 Jun 2016
DRR015410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10401340
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349460.3359759415613757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278850.2680904575756585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149890.14410643244043556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184550.058.9901921
GTATCAA350350.040.9854741
ATCAACG424500.033.2889333
TCAACGC425750.033.1805084
CAACGCA442000.031.9915265
AACGCAG458300.030.9549056
TATCAAC460050.030.8171162
ACGCAGA510500.027.3618777
CGCAGAG509650.027.0593368
GTGGTAT71800.025.7989861
GCAGAGT544050.024.6023039
GTACATG311250.023.893261
TGGTATC74800.023.182612
TACATGG305800.022.7120342
ACATGGG312700.021.4965423
CAGAGTA534550.021.03933510-11
GAGTACT370900.020.7177512-13
AGAGTAC504600.020.03404810-11
GTACTTT389700.019.36795414-15
TATAACG8800.019.1363032
Produced by FastQC (version 0.11.5)