FastQCFastQC Report
Thu 2 Jun 2016
DRR015410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10401340
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365720.35160854274545394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285820.2747915172468163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175980.1691897390143962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180100.058.3907471
GTATCAA368200.044.294961
ATCAACG463000.034.8073963
TCAACGC464800.034.6418954
CAACGCA477750.033.626985
TATCAAC491050.033.041872
AACGCAG495200.032.5785876
ACGCAGA556500.028.468527
CGCAGAG558000.028.1879868
GTGGTAT71200.026.8660051
GCAGAGT591150.025.8524239
TGGTATC72500.024.9220242
GTACATG300750.022.306471
TACATGG289050.021.4926762
AGAGTAC556150.021.26092710-11
GAGTACT415300.020.99754712-13
CAGAGTA584950.020.71988710-11
TATAACG9900.020.6875062
TAACGCA9700.020.1746774
AGTACTT423750.020.11699312-13
Produced by FastQC (version 0.11.5)