FastQCFastQC Report
Thu 2 Jun 2016
DRR015409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20738101
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT754410.36377969226786966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591420.2851852250116826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332340.16025575340770112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA369050.059.9481351
GTATCAA737600.043.7790871
TCAACGC907550.035.078574
ATCAACG908650.035.0511323
TATCAAC950300.033.6408042
CAACGCA960150.033.133165
AACGCAG1008950.031.6600516
ACGCAGA1086550.028.875527
CGCAGAG1080150.028.6801598
GCAGAGT1146150.026.1688829
GTACATG704150.025.9769421
GTGGTAT136000.025.7014451
TACATGG699200.024.6706372
ACATGGG731850.022.8227923
TGGTATC141700.022.8050522
GAGTACT744550.022.0788112-13
CAGAGTA1107100.021.9924110-11
AGAGTAC1052050.021.08270510-11
GTACTTT808150.020.0652714-15
CATGGGG471150.019.8024714
Produced by FastQC (version 0.11.5)