FastQCFastQC Report
Thu 2 Jun 2016
DRR015409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20738101
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT754710.36392435353651714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540030.26040474969236577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394320.19014277151027476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA345200.058.022781
GTATCAA714150.045.4688721
TCAACGC932200.034.3665664
ATCAACG933550.034.34673
TATCAAC973050.033.1262132
CAACGCA967200.033.0819475
AACGCAG1015550.031.5862776
ACGCAGA1134800.027.8995867
CGCAGAG1122350.027.794618
GTACATG658900.026.3410781
GCAGAGT1194250.025.156119
TACATGG659550.024.9811172
GTGGTAT130850.024.1545981
ACATGGG691150.023.028463
AGAGTAC1095850.021.7824210-11
TGGTATC140550.021.2433992
GAGTACT786050.020.72357412-13
AGTACTT824450.020.45670312-13
CAGAGTA1155600.020.27214810-11
CATGGGG443800.019.413224
Produced by FastQC (version 0.11.5)