FastQCFastQC Report
Thu 2 Jun 2016
DRR015408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7677267
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317020.4129334045565955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307480.40050710754230645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162250.21133822752289325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160800.062.268221
GTATCAA294300.038.8780821
TCAACGC355600.031.5743474
ATCAACG356650.031.5069123
CAACGCA366400.030.618825
TATCAAC370400.030.4851592
AACGCAG379400.029.6691326
GTGGTAT56550.027.8466741
ACGCAGA420300.026.3164967
CGCAGAG420100.026.1015858
TGGTATC56500.025.7771072
TATAACG7500.025.4871142
GCAGAGT447300.023.8206569
CAGAGTA435050.022.15937610-11
GTACATG221850.021.9304771
GAGTACT316200.021.92029412-13
TACATGG216700.020.7295992
GTACTTT339800.020.23037714-15
AGAGTAC413050.020.22254410-11
TAACGCA9250.020.1729374
Produced by FastQC (version 0.11.5)