FastQCFastQC Report
Thu 2 Jun 2016
DRR015408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7677267
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326070.42472145361103114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291750.3800180454841547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201800.2628539557110623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147950.059.4637641
GTATCAA278550.041.457811
ATCAACG364300.031.404293
TCAACGC365300.031.2811534
CAACGCA374300.030.5228335
TATCAAC380050.030.1870462
AACGCAG386100.029.6006226
GTGGTAT52550.029.1156731
ACGCAGA434000.026.0206747
CGCAGAG435900.025.6748738
TGGTATC57400.025.2117962
TAACGCA8750.024.4441814
TATAACG8150.024.0103972
GCAGAGT461050.023.5631059
AGAGTAC427250.021.88081410-11
GTACATG205050.021.4302791
GAGTACT331750.020.77798712-13
AGTACTT347100.020.60653112-13
TTTCGCG5100.020.5251249
CAGAGTA451400.020.3976210-11
Produced by FastQC (version 0.11.5)