FastQCFastQC Report
Thu 2 Jun 2016
DRR015407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997835
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254720.424686574405598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248670.414599601356156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137400.2290826606600548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124650.063.5989531
GTATCAA232650.039.9044381
GTGGTAT40550.032.9951821
TCAACGC292050.031.2732754
ATCAACG292550.031.2198263
TATCAAC303950.030.2293112
CAACGCA303400.030.133365
TGGTATC41750.029.9757672
AACGCAG314100.029.2738746
TATAACG5100.026.76982
ACGCAGA343000.026.5155417
CGCAGAG342550.026.284748
GCAGAGT362650.024.0363189
GTACATG165750.023.7770771
TACATGG163600.022.4205272
GAGTACT265100.021.96665812-13
CAGAGTA352200.021.92060910-11
AGAGTAC333000.021.10103610-11
ACATGGG167100.020.8065533
GTACTTT288100.020.04717414-15
Produced by FastQC (version 0.11.5)