FastQCFastQC Report
Thu 2 Jun 2016
DRR015407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997835
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256720.4280211109508681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229600.3828047953970058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164260.2738654864630321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114450.060.6752361
GTATCAA217450.041.2957081
ATCAACG287600.030.8901583
TCAACGC288450.030.813884
CAACGCA294750.030.1502325
TATCAAC303700.029.4026852
AACGCAG305900.029.1100356
GTGGTAT40400.027.6148151
ACGCAGA348550.025.105417
CGCAGAG345900.024.9991348
TATAACG6050.024.0728442
TGGTATC45600.023.5548292
GTACATG155650.023.550721
GCAGAGT366100.022.7862999
TACATGG156000.021.8519422
AGAGTAC338400.021.81563210-11
TAACGCA6750.021.57554
ACATGGG159600.020.474853
AGTACTT280700.020.35736712-13
GAGTACT271750.020.25739512-13
Produced by FastQC (version 0.11.5)