FastQCFastQC Report
Thu 2 Jun 2016
DRR015406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4511585
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165980.3678973132502214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155650.34500070374380626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.19620598969098446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89350.058.3219031
GTATCAA165100.039.943131
TCAACGC200100.032.45444
ATCAACG201050.032.233143
CAACGCA206750.031.3664995
TATCAAC209900.031.0499552
AACGCAG214400.030.3659156
TATAACG4350.028.2491192
ACGCAGA237550.027.157687
CGCAGAG236650.026.9533678
GTGGTAT34650.025.2184721
GCAGAGT252650.024.4876889
GTACATG128200.023.678741
TACATGG126850.022.7831442
TGGTATC35550.022.148122
CAGAGTA246150.021.76152210-11
GAGTACT176550.021.45062312-13
ACATGGG131250.021.2895133
AGAGTAC229400.021.12945210-11
TAACGCA5000.020.9341094
Produced by FastQC (version 0.11.5)