FastQCFastQC Report
Thu 2 Jun 2016
DRR015406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4511585
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164120.3637745936295116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145480.3224587367854091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101280.22448873289542368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87400.057.9455221
GTATCAA159650.039.7953071
ATCAACG209300.029.9431383
TCAACGC209850.029.8206214
CAACGCA215300.029.0368925
TATCAAC220250.028.5581172
AACGCAG223100.028.1641546
TATAACG4450.026.5919882
ACGCAGA248850.024.9409527
CGCAGAG249300.024.7017358
GTACATG122650.023.8764611
GTGGTAT33200.023.5948311
GCAGAGT263000.022.5323839
TACATGG124550.022.1445242
AGAGTAC239200.021.85523210-11
TAACGCA5300.021.467784
TGGTATC33850.021.3784352
ACATGGG126650.021.1302553
AGTACTT195950.020.23291812-13
ATAACGC6800.020.0791343
Produced by FastQC (version 0.11.5)