FastQCFastQC Report
Thu 2 Jun 2016
DRR015405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7966036
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289250.36310405827942527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273000.3427049538817048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124780.15664001518446566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211200.052.2163661
GTATCAA356450.035.5609781
ATCAACG420250.029.7177093
TCAACGC421800.029.6192974
CAACGCA441050.028.3781325
TATCAAC442800.028.3595562
AACGCAG451750.027.7469446
ACGCAGA483900.025.4803497
CGCAGAG482150.025.2519938
GTACATG276050.023.330061
GAGTACT301350.023.06777412-13
GCAGAGT512550.022.8912059
TACATGG273550.022.0295982
GTACTTT323000.021.09200514-15
CAGAGTA498250.021.01897610-11
ACATGGG283400.020.5243763
AGAGTAC439200.019.9605710-11
AGTACTT312350.018.82532912-13
GTGGTAT93250.018.3033071
TGGTATC92800.017.6567132
Produced by FastQC (version 0.11.5)