FastQCFastQC Report
Thu 2 Jun 2016
DRR015405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7966036
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294000.3690668734110667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254220.31912986584544684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148230.1860774919922531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191450.048.053141
GTATCAA342050.039.2255361
ATCAACG425650.031.2883593
TCAACGC429200.031.0278134
CAACGCA439100.030.289935
TATCAAC448200.029.8055632
AACGCAG450650.029.4921236
ACGCAGA502900.026.0218057
CGCAGAG499550.026.0024498
GCAGAGT524800.024.039789
GTACATG256500.022.798351
AGAGTAC451600.022.2932410-11
GAGTACT318600.022.16399412-13
TACATGG257250.021.7802942
AGTACTT330850.021.075112-13
GTACTTT337250.020.62026814-15
TATAACG7850.020.293622
ACATGGG264850.020.2788413
CAGAGTA517350.019.6447410-11
CATGGGG151250.017.8441494
Produced by FastQC (version 0.11.5)