FastQCFastQC Report
Thu 2 Jun 2016
DRR015404_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015404_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8496308
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334130.39326493342755464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275300.3240230933247712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149030.17540559970283565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178000.053.3881231
GTATCAA330750.040.4834021
ATCAACG398400.033.186273
TCAACGC399250.033.1498154
TATCAAC413850.032.0800742
CAACGCA412550.032.0259555
AACGCAG425450.031.1621176
ACGCAGA465850.027.805287
CGCAGAG467900.027.3917458
GCAGAGT491750.025.062249
GTACATG261750.024.4821831
TACATGG261350.023.1791442
GAGTACT321800.022.195712-13
ACATGGG268500.021.6799323
CAGAGTA477000.021.36888110-11
AGAGTAC433500.020.9000110-11
GTACTTT338500.020.87206614-15
GTGGTAT75550.019.8798771
AGTACTT329150.019.68209612-13
CATGGGG169000.018.1242494
Produced by FastQC (version 0.11.5)