FastQCFastQC Report
Thu 2 Jun 2016
DRR015404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8496308
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329630.38796851526568954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247620.2914442367202319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175950.2070899501289266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163000.051.5731541
GTATCAA312000.041.816691
ATCAACG392650.032.8583453
TCAACGC392550.032.8199584
CAACGCA403950.031.87595
TATCAAC410400.031.5375632
AACGCAG416000.030.9954056
ACGCAGA467550.027.151587
CGCAGAG470600.026.7489158
GCAGAGT495000.024.5931559
GTACATG241500.024.4569571
TACATGG240800.022.9061452
AGAGTAC445950.021.70713810-11
ACATGGG248300.021.3341083
GAGTACT336550.020.75097512-13
AGTACTT349600.020.39295812-13
CAGAGTA487900.019.81275710-11
GTACTTT358750.019.16167414-15
CCGGTCG10150.018.831249
CGCGTAT2500.018.20460512-13
Produced by FastQC (version 0.11.5)