FastQCFastQC Report
Thu 2 Jun 2016
DRR015403_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015403_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9771727
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431180.44125260560390195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395850.40509727707292686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205620.21042339803394017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216850.061.7286871
GTATCAA401650.039.979031
GTGGTAT69650.032.73921
ATCAACG485400.032.4520533
TCAACGC486650.032.3593444
TATCAAC507250.031.2345752
CAACGCA505300.031.1559935
AACGCAG519550.030.4310286
TGGTATC77150.028.0227872
ACGCAGA570050.027.240347
CGCAGAG570000.026.9074828
GCAGAGT603700.024.61269
GAGTACT414200.022.86253412-13
GTACATG294350.022.7612041
CAGAGTA586550.022.24133910-11
TACATGG290500.021.6528742
GTACTTT455000.020.6622514-15
AGAGTAC550500.020.65244310-11
ACATGGG297600.020.5391963
AGTACTT431800.019.33354412-13
Produced by FastQC (version 0.11.5)