FastQCFastQC Report
Thu 2 Jun 2016
DRR015403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9771727
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441000.4513020062881413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374230.3829722217986647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243790.24948507055098856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197750.059.5404321
GTATCAA383550.042.1099471
ATCAACG487550.032.768263
TCAACGC491300.032.4893574
CAACGCA503750.031.6869895
TATCAAC510900.031.4228882
AACGCAG519150.030.8251256
GTGGTAT70950.027.9899431
ACGCAGA584500.027.0299347
CGCAGAG584900.026.6750138
TGGTATC76850.024.7576242
GCAGAGT615400.024.5831669
AGAGTAC566450.022.02037610-11
GAGTACT435200.021.4921312-13
GTACATG273150.021.1105941
CAGAGTA601600.020.78295910-11
AGTACTT458750.020.42355312-13
TACATGG270750.020.341992
GTACTTT476200.019.50688414-15
ACATGGG275950.019.0509663
Produced by FastQC (version 0.11.5)