FastQCFastQC Report
Thu 2 Jun 2016
DRR015402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15796896
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543800.3442448440503755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418920.26519133885543084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239150.15139050101994722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA268850.055.884111
GTATCAA522350.042.6872981
TCAACGC614800.035.6976284
ATCAACG617500.035.5120543
CAACGCA636350.034.4386565
TATCAAC655200.033.6156462
AACGCAG657000.033.4417726
ACGCAGA731450.029.5900737
CGCAGAG735250.029.0844148
GCAGAGT786950.026.2922769
GTACATG454300.024.7861921
TACATGG450750.023.537822
ACATGGG460200.022.2328783
GAGTACT517950.021.79183412-13
CAGAGTA769100.021.5018810-11
AGAGTAC716750.021.1491110-11
GTGGTAT105350.020.1760061
GTACTTT562950.019.7629314-15
AGTACTT541850.019.50405912-13
CATGGGG286450.018.5424884
Produced by FastQC (version 0.11.5)