FastQCFastQC Report
Thu 2 Jun 2016
DRR015402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15796896
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534750.33851587045961434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380150.24064854259976137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281450.17816791349389147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243500.053.7762761
GTATCAA481900.044.119011
ATCAACG611750.034.194633
TCAACGC610950.034.1792644
CAACGCA626250.033.3271455
TATCAAC651500.032.332482
AACGCAG648600.032.2826276
ACGCAGA738350.027.9406387
CGCAGAG741050.027.4479068
GTACATG412200.024.8614441
GCAGAGT797350.024.6585379
TACATGG411400.023.0122532
AGAGTAC726450.021.9006410-11
TATAACG13500.021.5777572
ACATGGG423750.021.3315013
GAGTACT541350.020.09430512-13
AGTACTT569550.019.99441312-13
CAGAGTA776400.019.70903610-11
GTGGTAT104250.018.3058451
GTACTTT594100.018.15982414-15
Produced by FastQC (version 0.11.5)