FastQCFastQC Report
Thu 2 Jun 2016
DRR015401_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015401_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9964469
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378000.3793478608845087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347900.34914053122148303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179170.17980887892771807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199650.061.115781
GTATCAA368800.039.477461
TCAACGC447200.032.1591534
ATCAACG446550.032.1550063
CAACGCA458650.031.3166225
TATCAAC469900.030.6352752
AACGCAG475150.030.4315996
ACGCAGA530150.026.8539647
CGCAGAG528500.026.782848
GCAGAGT561350.024.4437989
GTGGTAT73600.024.4255921
GTACATG289650.023.8676741
TGGTATC73150.022.8961012
TACATGG285050.022.8479182
CAGAGTA547200.022.18257310-11
GAGTACT396600.022.0881712-13
ACATGGG286400.021.7068583
AGAGTAC516150.020.30413210-11
GTACTTT431650.020.0519914-15
AGTACTT412550.018.86320112-13
Produced by FastQC (version 0.11.5)