FastQCFastQC Report
Thu 2 Jun 2016
DRR015401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9964469
Sequences flagged as poor quality0
Sequence length97
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381280.382639556608586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325460.3266205153531011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212010.21276597879927167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183700.058.165021
GTATCAA348250.041.5105821
ATCAACG451350.031.6133463
TCAACGC453200.031.4436534
CAACGCA463200.030.7267045
TATCAAC477600.030.066852
AACGCAG480300.029.726146
ACGCAGA548700.025.6570937
CGCAGAG545550.025.5366698
GTGGTAT71900.023.944511
GTACATG272600.023.658131
GCAGAGT582200.023.2089799
TACATGG267700.022.4938812
TATAACG9550.021.9233742
TGGTATC74650.021.888582
ACATGGG270000.021.3410743
AGAGTAC533600.021.2982910-11
GAGTACT412800.020.7612112-13
CAGAGTA569900.020.04151510-11
AGTACTT429600.020.0022912-13
Produced by FastQC (version 0.11.5)