FastQCFastQC Report
Thu 2 Jun 2016
DRR015400_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015400_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11895083
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440340.37018657204829925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377310.3171982910922101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216620.18210885960190443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228450.059.408461
GTATCAA429000.041.109881
TCAACGC528250.033.0318874
ATCAACG531200.032.848453
CAACGCA560050.031.1319395
TATCAAC563250.031.0048542
AACGCAG578000.030.3290426
ACGCAGA630600.027.4889747
CGCAGAG631800.027.2135248
GTGGTAT76850.026.946311
GTACATG396900.024.998111
GCAGAGT676900.024.6187089
TACATGG398400.023.7505822
TGGTATC86200.022.9150892
ACATGGG418900.021.9571913
GAGTACT443000.021.58125512-13
CAGAGTA650750.021.5776910-11
AGAGTAC621700.020.98703610-11
GTACTTT475100.019.87407314-15
AGTACTT456450.019.68452312-13
Produced by FastQC (version 0.11.5)