FastQCFastQC Report
Thu 2 Jun 2016
DRR015400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11895083
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449980.3782907609808187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357510.3005527578075748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247570.20812801390288743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209550.060.294071
GTATCAA410100.043.103221
ATCAACG538350.032.581993
TCAACGC538800.032.5369264
CAACGCA557500.031.4540755
TATCAAC565800.031.162532
AACGCAG573300.030.6099856
ACGCAGA643400.026.9723537
CGCAGAG645000.026.5674888
GTGGTAT75250.024.3923911
GCAGAGT689100.024.0084299
GTACATG366600.023.6181281
TGGTATC76500.023.1432442
TTTCGCG12200.023.129889
TACATGG370400.022.3387572
AGAGTAC634500.021.69941710-11
GAGTACT468750.020.65773212-13
CAGAGTA663850.020.51036510-11
AGTACTT481950.020.45553212-13
ACATGGG393800.020.2020913
Produced by FastQC (version 0.11.5)