FastQCFastQC Report
Thu 2 Jun 2016
DRR015399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10394343
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415110.39936146036358433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350540.337241132027296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179380.172574639878634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217450.054.3707161
GTATCAA395000.039.9776951
TCAACGC467900.033.232474
ATCAACG469400.033.097513
CAACGCA482550.032.214125
TATCAAC490100.031.858212
AACGCAG498700.031.184946
ACGCAGA553150.027.761517
CGCAGAG554150.027.3974258
GCAGAGT588450.025.0723659
GTACATG304700.023.2392711
GTGGTAT90750.022.9669971
GAGTACT386150.022.37313512-13
TACATGG304200.021.9161262
CAGAGTA571450.021.72229610-11
GTACTTT412200.020.67766614-15
ACATGGG315900.020.3548283
AGAGTAC524650.020.19574410-11
TGGTATC95900.020.1202142
AGTACTT397000.018.97127712-13
Produced by FastQC (version 0.11.5)