FastQCFastQC Report
Thu 2 Jun 2016
DRR015399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10394343
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422730.4066923710329744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335050.32233879524660675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216600.20838257887006423No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210400.050.298241
GTATCAA386100.040.2648541
ATCAACG476850.032.1910863
TCAACGC478150.031.9878124
CAACGCA489650.031.248065
TATCAAC500100.030.7592372
AACGCAG507450.030.2054656
ACGCAGA571100.026.4576457
CGCAGAG574850.026.0355368
GCAGAGT609100.024.055719
TATAACG8800.023.7898042
GTACATG283900.022.3112641
GTGGTAT100450.021.578411
AGAGTAC537500.021.10188310-11
TACATGG281800.021.0597062
GAGTACT407850.020.78125812-13
TAACGCA10000.020.4782434
TGGTATC103250.020.0556642
AGTACTT422600.019.90517412-13
CAGAGTA592650.019.8407810-11
Produced by FastQC (version 0.11.5)