FastQCFastQC Report
Thu 2 Jun 2016
DRR015398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13681719
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489930.3580909679551232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397950.29086257362835766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216870.15851078362302282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243500.059.5416641
GTATCAA474450.042.0680431
ATCAACG579200.034.0270463
TCAACGC581350.033.915744
CAACGCA606100.032.5758445
TATCAAC608900.032.4876142
AACGCAG631000.031.387656
ACGCAGA687500.028.2853377
CGCAGAG685150.028.1017998
GTGGTAT90800.027.7650241
GCAGAGT728200.025.4018549
GTACATG408400.025.2380961
TGGTATC96350.024.7960052
TACATGG394150.024.3956342
ACATGGG422750.022.08813
CAGAGTA703350.021.78805710-11
GAGTACT493150.021.11192112-13
AGAGTAC670650.020.76786410-11
GTACTTT512050.019.96413614-15
AGTACTT498350.019.6272712-13
Produced by FastQC (version 0.11.5)