FastQCFastQC Report
Thu 2 Jun 2016
DRR015398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13681719
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488670.3571700310465374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359380.26267167159331367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256340.1873594977356281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227600.056.6617051
GTATCAA459250.044.2732051
ATCAACG593850.034.0035323
TCAACGC594500.033.9128884
CAACGCA613350.032.8250275
TATCAAC620500.032.624622
AACGCAG636900.031.6175086
ACGCAGA715250.027.6460867
CGCAGAG712250.027.3956268
GTGGTAT87900.025.6439531
GCAGAGT755700.025.0193359
GTACATG389700.023.9080331
TGGTATC93600.023.1448062
TACATGG379550.022.938682
AGAGTAC700400.021.54139110-11
AGTACTT528300.020.5873312-13
GAGTACT516850.020.47990412-13
ACATGGG408850.020.4148713
CAGAGTA732050.020.31167210-11
GTACTTT541700.019.28820614-15
Produced by FastQC (version 0.11.5)