FastQCFastQC Report
Thu 2 Jun 2016
DRR015397_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015397_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10099974
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380910.37713958471576264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325270.3220503339909588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174450.17272321691125145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195000.055.847941
GTATCAA359350.040.386481
ATCAACG433200.033.069543
TCAACGC434900.032.929484
CAACGCA449450.031.883715
TATCAAC451800.031.7395842
AACGCAG464250.031.0207356
ACGCAGA516500.027.3452327
CGCAGAG516100.027.0468888
GTGGTAT80950.026.029341
GCAGAGT544000.024.7793489
TGGTATC84150.023.4711672
GTACATG269600.023.3454041
TACATGG267000.021.8000932
GAGTACT375600.021.75409912-13
CAGAGTA527900.021.40727410-11
AGAGTAC487200.020.72103710-11
ACATGGG277250.020.106963
GTACTTT404350.019.91495914-15
AGTACTT389900.019.07181512-13
Produced by FastQC (version 0.11.5)