FastQCFastQC Report
Thu 2 Jun 2016
DRR015397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10099974
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374370.3706643205220132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289140.2862779646759487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210580.20849558622626158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177700.055.2005921
GTATCAA344350.042.3718951
ATCAACG445600.032.3867653
TCAACGC447750.032.2005964
CAACGCA460950.031.2201445
TATCAAC464250.031.1843592
AACGCAG479500.030.0872276
ACGCAGA545300.026.0242397
CGCAGAG543000.025.8792028
GCAGAGT571950.023.5983819
GTACATG265000.022.9930041
TACATGG260900.021.8575782
AGAGTAC516100.021.55042510-11
GTGGTAT77800.021.5403961
ACATGGG265900.020.3164983
GAGTACT400300.020.2481912-13
AGTACTT418250.019.90693312-13
CAGAGTA558300.019.64843810-11
TGGTATC79650.019.4275592
GTACTTT429200.018.61877614-15
Produced by FastQC (version 0.11.5)