FastQCFastQC Report
Thu 2 Jun 2016
DRR015396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7913878
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362880.4585362574454648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305410.3859169929079018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176640.2232028343120781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170350.058.423621
GTATCAA315100.041.0158541
ATCAACG387700.032.9232253
TCAACGC387700.032.816764
TATCAAC401200.031.7939132
CAACGCA403250.031.5964325
AACGCAG417500.030.6343986
GTGGTAT63450.027.6845511
ACGCAGA460300.027.3509967
CGCAGAG462700.026.9124248
GTACATG269550.025.2903181
TGGTATC66000.025.235912
GCAGAGT486550.024.6755899
TACATGG271450.023.6212562
GAGTACT319650.022.0746612-13
CAGAGTA466150.021.79321710-11
ACATGGG282250.021.7653263
AGAGTAC440250.020.64171410-11
GTACTTT343400.020.36903214-15
AGTACTT332700.019.30123312-13
Produced by FastQC (version 0.11.5)