FastQCFastQC Report
Thu 2 Jun 2016
DRR015396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7913878
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350390.4427538559477414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261860.33088708216123625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213990.2703984064449818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145250.057.1592981
GTATCAA289900.044.5056041
ATCAACG383750.033.302493
TCAACGC384750.033.2030564
TATCAAC394250.032.381122
CAACGCA394200.032.3426175
AACGCAG406000.031.3674056
ACGCAGA463050.027.0709617
CGCAGAG468250.026.5215478
GTGGTAT51800.026.2000961
GCAGAGT489950.024.3900839
GTACATG250300.024.1813471
TACATGG252050.022.5350532
TGGTATC59750.022.0135782
AGAGTAC447600.021.96579410-11
AGTACTT342100.021.0714512-13
ACATGGG264100.020.6795373
GAGTACT332400.020.25565312-13
CAGAGTA470250.020.094910-11
GTACTTT354100.018.73148314-15
Produced by FastQC (version 0.11.5)