FastQCFastQC Report
Thu 2 Jun 2016
DRR015395_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015395_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916731
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205420.4177979230509052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195130.3968693833362045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110660.22506824147995896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141100.044.990331
GTATCAA230350.033.6827961
ATCAACG274150.027.852673
TCAACGC275500.027.7156264
CAACGCA283900.026.9116065
TATCAAC285600.026.8481082
AACGCAG291850.026.2694116
GTACATG142350.025.8620571
TACATGG139100.024.5361332
ACGCAGA317100.023.7902167
CGCAGAG316900.023.5312378
ACATGGG141900.022.7366773
GAGTACT221350.022.09538312-13
TATAACG5600.021.940562
GCAGAGT331350.021.926799
AGAGTAC274100.020.7561710-11
GTACTTT239950.020.28793514-15
CAGAGTA325750.019.9441910-11
ATAACGC6900.019.7849663
AGTACTT227300.019.36529512-13
Produced by FastQC (version 0.11.5)