FastQCFastQC Report
Thu 2 Jun 2016
DRR015395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916731
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188950.38430005627722974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172500.35084286693740213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115150.23420032537879334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133650.039.32191
GTATCAA213400.032.864271
ATCAACG266900.025.9195463
TCAACGC266900.025.833764
GTACATG135600.025.2890761
CAACGCA273450.025.2251855
TATCAAC274600.025.1927432
AACGCAG282350.024.4937696
TACATGG134800.023.9042852
ACATGGG140800.022.2068253
ACGCAGA317200.021.5449477
CGCAGAG316450.021.4565938
AGAGTAC263350.021.04886610-11
TAACGCA6250.020.3892654
GCAGAGT328000.020.159929
GAGTACT207200.020.08690812-13
AGTACTT215000.019.7709512-13
TATAACG5400.019.385032
GTACTTT226800.018.18034414-15
CATGGGG85150.017.6915934
Produced by FastQC (version 0.11.5)