FastQCFastQC Report
Thu 2 Jun 2016
DRR015394_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015394_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13073589
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448370.34295861679604583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354370.27105793214089874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197920.151389186244114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225600.057.7926031
GTATCAA441450.042.8740121
TCAACGC547900.034.21024
ATCAACG547800.034.1671263
TATCAAC578700.032.5011442
CAACGCA588900.031.828445
AACGCAG614050.030.602016
ACGCAGA651050.028.2268987
CGCAGAG643800.028.1397848
GTGGTAT84250.025.8193151
GCAGAGT686050.025.4703969
GTACATG418250.025.438721
TACATGG408850.024.4875532
ACATGGG432200.022.668933
TGGTATC95900.021.5438822
CAGAGTA667250.021.429310-11
GAGTACT446250.021.42413712-13
AGAGTAC626250.020.8926410-11
GTACTTT465250.020.21193714-15
AGTACTT453450.019.59408612-13
Produced by FastQC (version 0.11.5)