FastQCFastQC Report
Thu 2 Jun 2016
DRR015394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13073589
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436860.33415460743029324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301340.23049523738278752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238030.18206936136664537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200350.057.869011
GTATCAA425950.045.6291961
ATCAACG563600.034.154833
TCAACGC568000.033.7784734
TATCAAC591300.032.7022932
CAACGCA592700.032.371915
AACGCAG617300.031.1991966
ACGCAGA683550.027.6177187
CGCAGAG677700.027.4842388
GCAGAGT715650.025.2255699
GTACATG406550.025.0569951
TACATGG398700.023.9245852
GTGGTAT76600.022.471911
ACATGGG418500.022.3024713
AGAGTAC660650.022.27391810-11
AGTACTT489000.020.65163412-13
GAGTACT470500.020.3367912-13
CAGAGTA694850.020.07730710-11
TGGTATC84950.019.1786942
GTACTTT497350.018.89995614-15
Produced by FastQC (version 0.11.5)