FastQCFastQC Report
Thu 2 Jun 2016
DRR015393_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015393_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405958
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416070.3998382465122385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344780.33132941724346765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186240.1789743914015413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200750.060.1858981
GTATCAA384350.041.784051
TCAACGC468150.033.9332584
ATCAACG468800.033.8768273
CAACGCA482050.032.9264645
TATCAAC485950.032.7483832
AACGCAG501250.031.8162126
ACGCAGA555050.028.2322737
GTGGTAT73350.028.2291011
CGCAGAG555900.027.917398
GCAGAGT586450.025.546159
TGGTATC76250.025.5440482
GTACATG302100.024.0870461
TACATGG300250.022.7197952
GAGTACT411300.022.06180812-13
CAGAGTA567900.021.99461710-11
ACATGGG306400.021.4012973
AGAGTAC537900.020.77696210-11
GTACTTT439000.020.35362214-15
AGTACTT422050.019.45175412-13
Produced by FastQC (version 0.11.5)