FastQCFastQC Report
Thu 2 Jun 2016
DRR015393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405958
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417640.4013469975565921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321040.3085155638721586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217700.2092070715641943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189850.056.9469681
GTATCAA370150.042.975541
ATCAACG474700.033.2016873
TCAACGC476900.032.951984
CAACGCA491050.031.9672645
TATCAAC496500.031.7076822
AACGCAG512150.030.7734436
GTGGTAT75850.029.1196821
ACGCAGA578200.026.8891357
TGGTATC80000.026.7956372
CGCAGAG581000.026.5596378
GCAGAGT614250.024.2921949
GTACATG285850.023.5789261
TACATGG286600.022.121172
TATAACG10750.021.5920982
AGAGTAC565050.021.28560610-11
GAGTACT434650.020.77740712-13
ACATGGG295950.020.545423
CAGAGTA596650.020.364210-11
AGTACTT451350.020.08425712-13
Produced by FastQC (version 0.11.5)